Protein phosphatase 1 is involved in the dissociation of Ca2+ calmodulin-dependent protein kinase II from postsynaptic densities

被引：45

作者：

Yoshimura, Y ^{[1
]}

Sogawa, Y ^{[1
]}

Yamauchi, T ^{[1
]}

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Dept Biochem, Tokushima 770, Japan

来源：

FEBS LETTERS | 1999年 / 446卷 / 2-3期

关键词：

Ca2+/calmodulin-dependent protein kinase II; postsynaptic density; translocation; protein phosphatase 1; autophosphorylation; dissociation;

D O I：

10.1016/S0014-5793(99)00226-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Autophosphorylation-dependent translocation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) to postsynaptic densities (PSDs) from cytosol may be a physiologically important process during synaptic activation. We investigated a protein phosphatase responsible for dephosphorylation of the kinase. CaM kinase II was shown to be targeted to two sites using the gel overlay method in two-dimensional gel electrophoresis. Protein phosphatase 1 (PP1) was identified to dephosphorylate CaM kinase Tr from its complex with PSDs using phosphatase inhibitors and activators, and purified phosphatases, The kinase was released from PSDs after its dephosphorylation by PP1. (C) 1999 Federation of European Biochemical Societies.

引用

页码：239 / 242

页数：4

共 30 条

[1]

BENNETT MK, 1983, J BIOL CHEM, V258, P2735

[2] THE MULTIFUNCTIONAL CALCIUM CALMODULIN-DEPENDENT PROTEIN-KINASE - FROM FORM TO FUNCTION [J].

BRAUN, AP ;

SCHULMAN, H .

ANNUAL REVIEW OF PHYSIOLOGY, 1995, 57 :417-445

[3] Autophosphorylation at Thr286 of the α calcium-calmodulin kinase II in LTP and learning [J].

Giese, KP ;

Fedorov, NB ;

Filipkowski, RK ;

Silva, AJ .

SCIENCE, 1998, 279 (5352) :870-873

[4]

GOLDENRING JR, 1983, J BIOL CHEM, V258, P2632

[5] IDENTIFICATION OF THE MAJOR POSTSYNAPTIC DENSITY PROTEIN AS HOMOLOGOUS WITH THE MAJOR CALMODULIN-BINDING SUBUNIT OF A CALMODULIN-DEPENDENT PROTEIN-KINASE [J].

GOLDENRING, JR ;

MCGUIRE, JS ;

DELORENZO, RJ .

JOURNAL OF NEUROCHEMISTRY, 1984, 42 (04) :1077-1084

[6] NEURONAL CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASES [J].

HANSON, PI ;

SCHULMAN, H .

ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :559-601

[7] TWO-DIMENSIONAL GEL-ELECTROPHORESIS OF CHICKEN SKELETAL-MUSCLE PROTEINS WITH AGAROSE GELS IN THE 1ST DIMENSION [J].

HIRABAYASHI, T .

ANALYTICAL BIOCHEMISTRY, 1981, 117 (02) :443-451

[8] A novel protein phosphatase that dephosphorylates and regulates Ca2+/calmodulin-dependent protein kinase II [J].

Ishida, A ;

Kameshita, I ;

Fujisawa, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (04) :1904-1910

[9] EVIDENCE THAT THE MAJOR POSTSYNAPTIC DENSITY PROTEIN IS A COMPONENT OF A CA-2+ CALMODULIN-DEPENDENT PROTEIN-KINASE [J].

KELLY, PT ;

MCGUINNESS, TL ;

GREENGARD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (03) :945-949

[10] BIOCHEMICAL AND IMMUNOCHEMICAL EVIDENCE THAT THE MAJOR POSTSYNAPTIC DENSITY PROTEIN IS A SUBUNIT OF A CALMODULIN-DEPENDENT PROTEIN-KINASE [J].

KENNEDY, MB ;

BENNETT, MK ;

ERONDU, NE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (23) :7357-7361

← 1 2 3 →