Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana

被引：390

作者：

Radakovits, Randor ^{[1
]}

Jinkerson, Robert E. ^{[1
]}

Fuerstenberg, Susan I. ^{[2
]}

Tae, Hongseok ^{[3
]}

Settlage, Robert E. ^{[3
]}

Boore, Jeffrey L. ^{[2
,4
]}

Posewitz, Matthew C. ^{[1
]}

机构：

[1] Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA

[2] Genome Project Solut, Hercules, CA 94547 USA

[3] Virginia Tech, Virginia Bioinformat Inst, Blacksburg, VA 24060 USA

[4] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA

来源：

NATURE COMMUNICATIONS | 2012年 / 3卷

基金：

美国国家科学基金会;

关键词：

PHAEODACTYLUM-TRICORNUTUM; THALASSIOSIRA-PSEUDONANA; NUCLEAR TRANSFORMATION; C-4; PHOTOSYNTHESIS; BIOFUEL PRODUCTION; CHLAMYDOMONAS; ASSIMILATION; EVOLUTION; REVEALS; MECHANISMS;

D O I：

10.1038/ncomms1688

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (similar to 29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.

引用

页数：10

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