HIV interaction with endosornes in macrophages and dendritic cells

The human immunodeficiency virus type I (HIV-1) is an enveloped retrovirus that undergoes assembly at specific sites in infected cells. In macrophages, at least, this assembly occurs primarily on a subset of endocytic organelles that contain some of the markers found in late endosomes or multivesicular bodies (MVBs), in particular CD63. The budding of virus particles into endosomes has many features in common with the formation of exosomes and some limited biochemical comparison of HIV-1 particles produced from macrophages with exosomes suggests that the two have similar cellular origins. Here we show that macrophages infected with HIV contain large intracellular pools of infectious virus that can be released by homogenisation of intact cells. Immunoprecipitation experiments indicate this virus has a similar complement of cellular membrane proteins to viruses that can be recovered from the extracellular medium, further suggesting that viruses released from macrophages initially bud into endosomal organelles and are then released by fusion of these organelles with the plasma membrane. (c) 2005 Elsevier Inc. All rights reserved.