Rapid liquid-liquid extraction of cocaine from urine for gas chromatographic mass spectrometric analysis

被引：11

作者：

Garside, D

Goldberger, BA

Preston, KL

Cone, EJ

机构：

[1] UNIV FLORIDA,COLL MED,DIAGNOST REFERRAL LABS,GAINESVILLE,FL 32608

[2] NIDA,ADDICT RES CTR,BALTIMORE,MD 21224

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 692卷 / 01期

关键词：

cocaine;

D O I：

10.1016/S0378-4347(96)00459-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel, simple and economic liquid-liquid extraction method for isolating cocaine from urine was developed utilizing gas chromatography-mass spectrometry (CC-MS) for analysis and quantification. The use of a single nonpolar organic solvent allowed only nonpolar analytes to be extracted from the biological fluid, and consequently, no derivatization step was necessary before CC-MS analysis. Large numbers of specimens (>60) can be extracted in approximately 3 h with this procedure. The method is highly precise (C.V. <7%), accurate (>98%), sensitive (limit of detection of 5 ng/mI) and has a mean recovery of 48.8%.

引用

页码：61 / 65

页数：5

共 25 条

[1] DETERMINATION OF COCAINE AND BENZOYLECGONINE BY DERIVATIZATION WITH IODOMETHANE-D(3) OR PFPA/HFIP IN HUMAN BLOOD AND URINE USING GC/MS (EI OR PCI MODE) [J].