Characterization of a new peptide from Tityus serrulatus scorpion venom which is a ligand of the apamin-binding site

被引：48

作者：

Legros, C

Oughuideni, R

Darbon, H

Rochat, H

Bougis, PE

MartinEauclaire, MF

机构：

[1] FAC MED NORD,INST FEDERAT JEAN ROCHE,URA 1455 CNRS,LAB BIOCHIM INGN PROT,F-13916 MARSEILLE 20,FRANCE

[2] CNRS,URA 1296,UPR 9039,INST FEDERAT RECH,IFRC 1,F-13008 MARSEILLE,FRANCE

来源：

FEBS LETTERS | 1996年 / 390卷 / 01期

关键词：

scorpion toxin; cDNA; amino acid sequence; apamin; potassium channels;

D O I：

10.1016/0014-5793(96)00616-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new ligand (Ts k) of the apamin binding site on rat brain synaptosomes (K-0.5 = 300 PM) was purified and characterized from the venom of Tityus serulatus. It is a polypeptide toxin of 35 amino acid residues, with three disulfide bridges, Its cDNA was amplified from a venom gland cDNA library and the nucleotide sequence determined, A model of Ts Ic was constructed by amino acid replacement using charybdotoxin structure as determined by H-1 nuclear magnetic resonance as starting model.

引用

页码：81 / 84

页数：4

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