Differential role of cytosolic phospholipase A2 in the invasion of brain microvascular endothelial cells by Escherichia coli and Listeria monocytogenes

被引:32
作者
Das, A
Asatryan, L
Reddy, MA
Wass, CA
Stins, MF
Joshi, S
Bonventre, JV
Kim, KS
机构
[1] Childrens Hosp Los Angeles, Div Infect Dis, Los Angeles, CA 90027 USA
[2] Childrens Hosp Los Angeles, Div Hematol & Oncol, Los Angeles, CA 90027 USA
[3] Massachusetts Gen Hosp, Dept Med, Charlestown, MA USA
关键词
D O I
10.1086/322986
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Invasion of brain microvascular endothelial cells (BMECs) is a key step in the pathogenesis of meningitis due to Escherichia coli and Listeria monocytogenes. Although host cell actin cytoskeletal rearrangements are essential in BMEC invasion by E. coli K1 and L. monocytogenes, the underlying signaling mechanisms remain unclear. This study demonstrates that host cell cytosolic phospholipase A(2) (cPLA(2)) contributes to E. coli K1 invasion of BMECs but not to L. monocytogenes invasion of BMECs. This difference was observed with 4-bromophenacyl bromide, a nonselective PLA(2) inhibitor, and arachidonyl trifluoromethyl ketone, a selective cPLA(2) inhibitor, and was confirmed with BMEC derived from cPLA(2) knockout mice. Activation of cPLA(2) leads to generation of intracellular arachidonic acid, which is metabolized via cyclooxygenase (COX) and lipo-oxygenase (LOX) pathways into eicosanoids. COX and LOX inhibitors also significantly inhibit E. coli K1 invasion of BMECs.
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页码:732 / 737
页数:6
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