Rubber stoppers as sources of contaminants in electrospray analysis of peptides and proteins

被引：14

作者：

Zhang, XK ^{[1
]}

Dutky, RC ^{[1
]}

Fales, HM ^{[1
]}

机构：

[1] NHLBI,CHEM STRUCT SECT,BIOPHYS CHEM LAB,NIH,BETHESDA,MD 20892

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 18期

关键词：

D O I：

10.1021/ac960245n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Using MS/MS, we have identified ions at m/z 399, 421, 609.4, and 819.9 as those of tris(2-butoxyethyl) phosphate, originating in the gray rubber stoppers commonly used as closures in commercial sources of peptides and proteins. The compound is readily extracted by contact with organic solvents such as alcohol or acetic acid and even by water alone, Zinc salts of proteins are also observed, presumably from zinc oxide fillers present in the stoppers.

引用

页码：3288 / 3289

页数：2

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