Effect of genotype on the levels of surfactant protein A mRNA and on the SP-A2 splice variants in adult humans

被引:69
作者
Karinch, AM
deMello, DE
Floros, J
机构
[1] PENN STATE UNIV,COLL MED,DEPT CELLULAR & MOL PHYSIOL,HERSHEY,PA 17033
[2] PENN STATE UNIV,COLL MED,DEPT PAEDIAT,HERSHEY,PA 17033
[3] ST LOUIS UNIV,HLTH SCI CTR,ST LOUIS,MO 63104
[4] CARDINAL GLENNON CHILDRENS HOSP,DEPT PATHOL & LAB MED,ST LOUIS,MO
关键词
D O I
10.1042/bj3210039
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human pulmonary surfactant protein A (SP-A) is encoded by two genes, SP-A1 and SP-A2, that exhibit coding sequence (allelic) and 5' splicing variability. In this report we determine the effect of the genetic variability within the SP-A1 and SP-A2 genes on the level of SP-A mRNAs and on the SP-A2 splicing variants in different individuals. We analysed mRNA specimens from 23 unrelated adults using genotype analysis, Northern analysis and primer extension, and made the following observations. (1) The level of SP-A mRNA varies among individuals (coefficient of variation = 0.49). One SP-A genotype (6A(2)6A(2)1A(0)1A(0)) appears to be associated with a low to moderate level of SP-A mRNA. (2) The SP-A1/SP-A2 mRNA ratio varies among individuals, from 0.94 (lowest) to 6.80 (highest) within the study population. One genotype appears to be associated with a moderate to high SP-A1/SP-A2 mRNA ratio and another with a low to moderate ratio. (3) There is no correlation between the level of SP-A mRNA and the SP-A1/SP-A2 mRNA ratio. (4) Variability in the ratio of the major SP-A2 splice variants among individuals results from nucleotide differences in the splice-recognition sequence of specific SP-A2 alleles. The SP-A mRNA levels, the SP-A1/SP-A2 mRNA ratio, and the ratio of the major SP-A2 splice variants have a genetic basis in that they vary depending upon the specific SP-A alleles present.
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页码:39 / 47
页数:9
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