Innovative micropropagation of temperate fruit trees: The case of pear

被引：10

作者：

Damiano, C ^{[1
]}

Caboni, E ^{[1
]}

Frattarelli, A ^{[1
]}

Giorgioni, M ^{[1
]}

Liberali, M ^{[1
]}

Lauri, P ^{[1
]}

D'Angeli, S ^{[1
]}

机构：

[1] Ist Sperimentale Frutticoltura, I-00040 Rome, Italy

来源：

PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON METHODS AND MARKERS FOR QUALITY ASSURANCE IN MICROPROPAGATION | 2000年 / 530期

关键词：

micropropagation; Pyrus communis var pyraster; temporary immersion;

D O I：

10.17660/ActaHortic.2000.530.20

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Within plant micropropagation methods, new techniques are being assessed in order to increase the multiplication rate and automate plant production as much as possible. In this context, the temporary immersion technique, a method based on the complete immersion of the proliferating explants in a liquid medium, alternating with a dried period, has been recently acquiring importance. In the present work this method has been applied to in vitro culture of wild pear (Pyrus communis var pyraster L.), in comparison to the solid medium culture, to improve proliferation ability of shoots obtained from leaves through adventitious regeneration. With temporary immersion, the multiplication rates were much higher (x 13) than with solid culture medium (x 4 - x 5), moreover, the plants revealed an excellent stem elongation and a higher rooting ability.

引用

页码：181 / 185

页数：5

共 10 条

[1]

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[2]

ALVAREZ R, 1993, AGROCHIMICA, V37, P55

[3]

CABONI E, 2000, IN PRESS PLANT CELL

[4] Pineapple (Ananas comosus L-Merr) micropropagation in temporary immersion systems [J].