Determination of Adenosine Phosphates in Rat Gastrocnemius at Various Postmortem Intervals Using High Performance Liquid Chromatography

被引：12

作者：

Huang, Hong ^{[1
]}

Yan, Youyi ^{[1
]}

Zuo, Zhong ^{[2
]}

Yang, Lin ^{[1
]}

Song, Yu ^{[1
]}

Liao, Linchuan ^{[1
]}

机构：

[1] Sichuan Univ, W China Preclin Med & Forens Med Sch, Chengdu 610014, Sichuan, Peoples R China

[2] Chinese Univ Hong Kong, Fac Med, Sch Pharm, Shatin, Hong Kong, Peoples R China

来源：

JOURNAL OF FORENSIC SCIENCES | 2010年 / 55卷 / 05期

关键词：

forensic science; rigor mortis; adenosine triphosphate; adenosine diphosphate; adenosine monophosphate; high performance liquid chromatography; RIGOR-MORTIS; NUCLEOTIDES; BREAKING;

D O I：

10.1111/j.1556-4029.2010.01450.x

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

030101 [法学理论]; 030111 [法律史学]; 100218 [急诊医学];

摘要：

Although the change in adenosine phosphate levels in muscles may contribute to the development of rigor mortis, the relationship between their levels and the onset and development of rigor mortis has not been well elucidated. In the current study, levels of the adenosine phosphates including adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) in gastrocnemius at various postmortem intervals of 180 rats from different death modes were detected by high performance liquid chromatography. The results showed that the levels of ATP and ADP significantly decreased along with the postmortem period of rats from different death mode whereas the AMP level remained the same. In addition, it was found that changes in the ATP levels in muscles after death correlated well with the development of rigor mortis. Therefore, the ATP level could serve as a reference parameter for the deduction of rigor mortis in forensic science.

引用

页码：1362 / 1366

页数：5

共 14 条

[1]

[Anonymous], 2001, Forensic Pathology

[2]

Differential rigor development in red and white muscle revealed by simultaneous measurement of tension and stiffness [J].