Species-level identification of clinical staphylococcal isolates based on polymerase chain reaction-restriction fragment length polyrnorphism analysis of a partial groEL gene sequence

被引：19

作者：

Barros, Elaine Menezes ^{[1
]}

Iorio, Natalia Lopes Pontes ^{[1
]}

de Freire Bastos, Maria do Carmo ^{[1
]}

dos Santos, Katia Regina Netto ^{[1
]}

Giambiagi-deMarval, Marcia ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Ctr Ciencias Saude, Inst Microbiol Prof Paulo Goes, Dept Microbiol Med, BR-21941920 Rio De Janeiro, Brazil

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2007年 / 59卷 / 03期

关键词：

staphylococcus; groEL; HSP60; PCR-RFLP;

D O I：

10.1016/j.diagmicrobio.2007.05.004

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A pair of degenerate primers that amplified, by polymerase chain reaction (PCR), a partial groEL gene sequence (550 bp) was used for the identification of the 12 most common human stapbylococcal pathogens. The amplified products were digested by AluI endonuclease, and distinctive PCR restriction fragment length polymorphism (RFLP) patterns for reference strains were obtained. This protocol was validated by the identification of 89 clinical staphylococcal isolates, and the results were compared with those obtained by the reference biochemical identification, showing 100% concordant results. Two species, Staphylococcus aureus and Staphylococcus lugdunensis, showed intraspecies polymorphisms on their PCR-RFLP patterns. All strains were also identified using the API Staph ID test (bioMerieux, Durham, NC) and the MicroScan WalkAway automated system (Dade Behring, West Sacramento, CA). When 17 Staphylococcus isolates were tested in a blind experiment by the PCR-RFLP of the groEL gene method, all strains were also correctly identified. We propose the PCR-RFLP of the groEL gene with AluI as a reliable and reproducible method for identification of Staphylococcus spp. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：251 / 257

页数：7

共 39 条

[1] Comparison of two commercial methods with PCR restriction fragment length polymorphism of the tuf gene in the identification of coagulase-negative staphylococci [J].