A preliminary study of prostaglandin release by bradykinin (BK) an isolated human myometrium

The aim of this study was to investigate whether the response to BK on isolated human myometrium from non-pregnant (NP) and pregnant (P) donors involves the release of prostaglandin I-2 (PGI(2)) and/or of PGE(2). BK was injected as a bolus dose into the now of the superfusate. The perfusate was collected during a response to BK and PG concentration measured by enzymoimmnunoassay for PGE(2) or PGI(2). The BK response was biphasic, consisting of contraction followed by inhibition of myogenic activity. In tissues from both NP and P donors BK was found to cause a dose related release of PGE(2) and PGI(2). BK evoked PGE(2) release which was greater during the contractile response than in the inhibitory response. Following the satire dose of BK, PGI(2) release was found to be greater in the inhibitory response than in findings indicate that in isolated human myometrium the response to BK does involve the release of PGE(2) and PGI(2) and in both NP and P tissue PGE(2) release is greater in the contractile response phase whilst PGI(2) predominates the inhibitory phase.