Activation of Serratia marcescens hemolysin through a conformational change

被引：33

作者：

Walker, G ^{[1
]}

Hertle, R ^{[1
]}

Braun, V ^{[1
]}

机构：

[1] Univ Tubingen, D-72074 Tubingen, Germany

来源：

INFECTION AND IMMUNITY | 2004年 / 72卷 / 01期

关键词：

D O I：

10.1128/IAI.72.1.611-614.2004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

For Serratia marcescens, secreted hemolysin/cytotoxin is not only secreted but also activated by an outer membrane protein. Excluding posttranslational processing by mass spectrometry, the conformation of active and inactive ShlA derivatives strongly differed in electrophoretic mobilities, gel permeation chromatography, sensitivity to trypsin, circular dichroism, and intrinsic fluorescence. We concluded that ShlB interacts with ShlA during secretion and imposes a conformational change in ShlA to form the active hemolysin.

引用

页码：611 / 614

页数：4

共 35 条

[1] GENES ENCODING HIGH-MOLECULAR-WEIGHT ADHESION PROTEINS OF NONTYPABLE HAEMOPHILUS-INFLUENZAE ARE PART OF GENE CLUSTERS [J].