Validation of a simple, sensitive method for the determination of β-estradiol in bovine urine using gras-chromatography negative ion chemical ionization mass spectrometry

被引：8

作者：

Akre, C ^{[1
]}

Fedeniuk, R ^{[1
]}

MacNeil, JD ^{[1
]}

机构：

[1] Canadian Food Inspect Agcy, Saskatoon Lab, Ctr Vet Drug Residues, Saskatoon, SK S7N 2R3, Canada

来源：

ANALYST | 2004年 / 129卷 / 02期

关键词：

D O I：

10.1039/b313274a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method is presented for the analysis of 17beta-estradiol in bovine urine. After deconjugation, the sample is cleaned up using an OASIS(TM) HLB disposable cartridge and extracted into 1-chlorobutane. The hormone is derivatized using pentafluorobenzoyl chloride. The derivatized estradiol is quantitated using gas-chromatography negative-ion chemical ionization mass spectrometry. Calibrations, obtained using spiked blank urine, are linear in the range of 100-1000 pg mL(-1) with CCalpha approximately 170 pg mL(-1) and CCbeta of 287 pg mL(-1). Recoveries, are in the range of 80 to 130%. The method is rugged, rapid and sensitive when compared to other hormone methods.

引用

页码：145 / 149

页数：5

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