Rapid detection of Betacoronavirus 1 from clinical fecal specimens by a novel reverse transcription loop-mediated isothermal amplification assay

被引：6

作者：

Qiao, Jun ^{[1
]}

Meng, Qingling ^{[1
]}

Cai, Xuepeng ^{[2
]}

Chen, Chuangfu ^{[1
]}

Zhang, Zaichao ^{[1
]}

Tian, Zhenzhong ^{[1
]}

机构：

[1] Shihezi Univ, Coll Anim Sci & Technol, Shihezi City 832003, Xinjiang, Peoples R China

[2] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2012年 / 24卷 / 01期

关键词：

Betacoronavirus; 1; nucleocapsid gene; rapid detection; reverse transcription loop-mediated isothermal amplification; NEONATAL CALF DIARRHEA; BOVINE CORONAVIRUS; PCR ASSAY; CATTLE; FECES; GENE; DNA;

D O I：

10.1177/1040638711425937

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Betacoronavirus 1 (BCoV-1) is an important pathogen causing diarrhea in calves. In the current study, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of BCoV-1 was successfully developed. The primers were designed to target the highly conserved fragment of BCoV-1 nucleocapsid gene. The assay displayed high specificity detecting only BCoV-1 with no cross reaction with other viruses. When 418 clinical samples from 6 different geographical areas of Xinjiang province were tested by the RT-LAMP method, the results indicated that this test is a simple, rapid, accurate, and sensitive method for the detection of BCoV-1.

引用

页码：174 / 177

页数：4

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