Observation and quantitative analysis of rat bone marrow stromal cells cultured in vitro on newly formed transparent β-tricalcium phosphate

被引：26

作者：

Kotobuki, N

Kawagoe, D

Nomura, D

Katou, Y

Muraki, K

Fujimori, H

Goto, S

Ioku, K

Ohgushi, H

机构：

[1] Natl Inst Adv Ind Sci & Technol, Res Inst Cell Engn, Amagasaki, Hyogo 6610974, Japan

[2] Tohoku Univ, Grad Sch Environm Studies, Aoba Ku, Sendai, Miyagi 9808579, Japan

[3] Yamaguchi Univ, Div Appl Med Engn Sci, Grad Sch Med, Ube, Yamaguchi 7558611, Japan

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2006年 / 17卷 / 01期

关键词：

D O I：

10.1007/s10856-006-6327-1

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

To observe living cell morphology on ceramics by light microscopy, we fabricated a new material-transparent beta - tricalcium phosphate (t-beta TCP) ceramic-for the purpose of serving as a tissue culture substrate. Bone marrow stromal cells (BMSCs) were obtained from rat femora and cultured on both t-beta TCP ceramic disks and culture grade polystyrene (PS) dishes in an osteogenic medium. After 1 day of culture, cell attachment and spreading on both the t-beta TCP and PS substrata were equally and clearly detected by ordinary light microscopy. After 14 days of culture, extensive cell growth, alkaline phosphatase (ALP) staining, and bone mineral deposition could be detected on both substrata. In addition, quantitative biochemical analyses revealed high DNA content, ALP activity, and osteocalcin content of these cultures. This experiment is significant in that all of the results were similarly observed on both the t-beta TCP and PS substrata, indicating the excellent properties of beta TCP ceramics for BMSCs culture towards osteogenic differentiation.

引用

页码：33 / 41

页数：9

共 26 条

[1]

[Anonymous], BIOMAT RES

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