Size-dependent DNA mobility in nanochannels

被引：84

作者：

Cross, Joshua David ^{[1
]}

Strychalski, Elizabeth A.

Craighead, H. G.

机构：

[1] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14853 USA

[2] Cornell Univ, Dept Phys, Ithaca, NY 14853 USA

来源：

JOURNAL OF APPLIED PHYSICS | 2007年 / 102卷 / 02期

关键词：

D O I：

10.1063/1.2757202

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Nanofluidic slits are used to investigate surface interactions during electrophoresis between DNA molecules and channel walls. The channels have vertical dimensions of 19 and 70 nm and contain no sieving matrix. Size-dependent mobility is observed for DNA in the 19 nm channels. We present a model for double stranded DNA mobility in the nanochannels that accurately predicts the size dependence of the DNA mobility in the range of 2000-10 000 bp. Due to surface interactions, the DNA mobility in the nanochannels scales as N-1/2. These results suggest that the notion of free solution DNA electrophoresis breaks down due to surface interactions in nanoscale environments.

引用

页数：5

共 31 条

[1] Toward single molecule DNA sequencing:: Direct identification of ribonucleoside and deoxyribonucleoside 5′-monophosphates by using an engineered protein nanopore equipped with a molecular adapter [J].

Astier, Y ;

Braha, O ;

Bayley, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (05) :1705-1710

[2] Electrohydrodynamic stretching of DNA in confined environments [J].

Bakajin, OB ;

Duke, TAJ ;

Chou, CF ;

Chan, SS ;

Austin, RH ;

Cox, EC .

PHYSICAL REVIEW LETTERS, 1998, 80 (12) :2737-2740

[3] Double-stranded DNA diffusion in slitlike nanochannels [J].

Balducci, Anthony ;

Mao, Pan ;

Han, Jongyoon ;

Doyle, Patrick S. .

MACROMOLECULES, 2006, 39 (18) :6273-6281

[4]

BROCHARD F, 1977, J PHYS-PARIS, V38, P1285, DOI 10.1051/jphys:0197700380100128500

[5]

Cabodi M, 2002, ELECTROPHORESIS, V23, P3496, DOI 10.1002/1522-2683(200210)23:20<3496::AID-ELPS3496>3.0.CO

[6]

2-9

[7] Entropic recoil separation of long DNA molecules [J].

Cabodi, M ;

Turner, SWP ;

Craighead, HG .

ANALYTICAL CHEMISTRY, 2002, 74 (20) :5169-5174

[8] Nanofilter array chip for fast gel-free biomolecule separation [J].

Fu, JP ;

Mao, P ;

Han, JY .

APPLIED PHYSICS LETTERS, 2005, 87 (26) :1-3

[9] A matrix for DNA separation: Genotyping and sequencing using poly(vinylpyrrolidone) solution in uncoated capillaries [J].

Gao, QF ;

Yeung, ES .

ANALYTICAL CHEMISTRY, 1998, 70 (07) :1382-1388

[10]

GROSSMAN P, 1992, CAPILLARY ELECTROPHO, P13303

← 1 2 3 4 →