Optogenetic experimentation on astrocytes

被引：61

作者：

Figueiredo, M. ^{[1
]}

Lane, S. ^{[1
]}

Tang, F. ^{[1
]}

Liu, B. H. ^{[1
]}

Hewinson, J. ^{[1
]}

Marina, N. ^{[2
]}

Kasymov, V. ^{[2
]}

Souslova, E. A. ^{[3
]}

Chudakov, D. M. ^{[3
]}

Gourine, A. V. ^{[2
]}

Teschemacher, A. G. ^{[1
]}

Kasparov, S. ^{[1
]}

机构：

[1] Univ Bristol, Sch Med Sci, Sch Physiol & Pharmacol, Bristol BS8 1TD, Avon, England

[2] UCL, London WC1E 6BT, England

[3] Russian Acad Sci, Shemiakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

EXPERIMENTAL PHYSIOLOGY | 2011年 / 96卷 / 01期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

GREEN FLUORESCENT PROTEIN; IN-VIVO; LENTIVIRAL VECTORS; CA2+ SENSOR; NEURAL ACTIVITY; LIVING CELLS; RAT-BRAIN; INDICATORS; CHANNELRHODOPSIN-2; EXPRESSION;

D O I：

10.1113/expphysiol.2010.052597

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We briefly review the current literature where optogenetics has been used to study various aspects of astrocyte physiology in vitro and in vivo. This includes both genetically engineered Ca2+ sensors and effector proteins, such as channelrhodopsin. We demonstrate how the ability to target astrocytes with cell-specific viral vectors to express optogenetic constructs helped to unravel some previously unsuspected roles of these inconspicuous cells.

引用

页码：40 / 50

页数：11

共 63 条

[1] Photostimulation of channelrhodopsin-2 expressing ventrolateral medullary neurons increases sympathetic nerve activity and blood pressure in rats [J].

Abbott, Stephen B. G. ;

Stornetta, Ruth L. ;

Socolovsky, Carmela S. ;

West, Gavin H. ;

Guyenet, Patrice G. .

JOURNAL OF PHYSIOLOGY-LONDON, 2009, 587 (23) :5613-5631

[2] Temporally precise in vivo control of intracellular signalling [J].

Airan, Raag D. ;

Thompson, Kimberly R. ;

Fenno, Lief E. ;

Bernstein, Hannah ;

Deisseroth, Karl .

NATURE, 2009, 458 (7241) :1025-1029

[3] Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters [J].

Amendola, M ;

Venneri, MA ;

Biffi, A ;

Vigna, E ;

Naldini, L .

NATURE BIOTECHNOLOGY, 2005, 23 (01) :108-116

[4] Tripartite synapses: glia, the unacknowledged partner [J].

Araque, A ;

Parpura, V ;

Sanzgiri, RP ;

Haydon, PG .

TRENDS IN NEUROSCIENCES, 1999, 22 (05) :208-215

[5] Circular permutation and receptor insertion within green fluorescent proteins [J].

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11241-11246

[6] Spectral characteristics of the photocycle of channelrhodopsin-2 and its implication for channel function [J].

Bamann, Christian ;

Kirsch, Taryn ;

Nagel, Georg ;

Bamberg, Ernst .

JOURNAL OF MOLECULAR BIOLOGY, 2008, 375 (03) :686-694

[7] Locus Coeruleus α-Adrenergic-Mediated Activation of Cortical Astrocytes In Vivo [J].

Bekar, Lane K. ;

He, Wei ;

Nedergaard, Maiken .

CEREBRAL CORTEX, 2008, 18 (12) :2789-2795

[8] Adenoviral vectors for highly selective gene expression in central serotonergic neurons reveal quantal characteristics of serotonin release in the rat brain [J].

Benzekhroufa, Kheira ;

Liu, Beihui ;

Tang, Feige ;

Teschemacher, Anja G. ;

Kasparov, Sergey .

BMC BIOTECHNOLOGY, 2009, 9

[9] Protoplasmic astrocytes in CA1 stratum radiatum occupy separate anatomical domains [J].

Bushong, EA ;

Martone, ME ;

Jones, YZ ;

Ellisman, MH .

JOURNAL OF NEUROSCIENCE, 2002, 22 (01) :183-192

[10] GLUTAMATE INDUCES CALCIUM WAVES IN CULTURED ASTROCYTES - LONG-RANGE GLIAL SIGNALING [J].

CORNELLBELL, AH ;

FINKBEINER, SM ;

COOPER, MS ;

SMITH, SJ .

SCIENCE, 1990, 247 (4941) :470-473

← 1 2 3 4 5 6 7 →