Enhancement of inosine production by Bacillus subtilis through suppression of carbon overflow by sodium citrate

被引：36

作者：

Chen, SX

Chu, J

Zhuang, YP

Zhang, SL

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] Henan Univ, Sch Life Sci, Kaifeng 475001, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 10期

关键词：

Bacillus subtilis; carbon overflow; inosine; sodium citrate;

D O I：

10.1007/s10529-005-4686-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sodium citrate, added to culture medium at 2 g l(-1), increased inosine production by B. subtilis by 18% to 16 g l(-1). The phosphoenolpyruvate (PEP) pool was increased by 2.3-fold, while the pyruvate and acetate pools were decreased by 78% and 57%, respectively. Pyruvate kinase might thus be a regulatory site for inosine synthesis.

引用

页码：689 / 692

页数：4

共 13 条

[1] Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the actinomycete Streptomyces coelicolor A 3(2) [J].