Thermotoga maritima phosphofructokinases:: Expression and characterization of two unique enzymes

被引：24

作者：

Ding, YHR ^{[1
]}

Ronimus, RS ^{[1
]}

Morgan, HW ^{[1
]}

机构：

[1] Univ Waikato, Thermophile Res Unit, Dept Biol Sci, Hamilton, New Zealand

来源：

JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 02期

关键词：

D O I：

10.1128/JB.183.2.791-794.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A pyrophosphate-dependent phosphofructokinase (PPi-PFK) and an ATP-dependent phosphofructokinase (ATP-PFK) from Thermotoga maritima have been cloned and characterized. The PPi-PFK is unique in that the K-m and V-max values indicate that polyphosphate is the preferred substrate over pyrophosphate; the enzyme in reality Is a polyphosphate-dependent PFK. The ATP-PFK was not significantly affected by common allosteric effecters (e.g., phosphoenolpyruvate) but was strongly inhibited by PPi and polyphosphate. The results suggest that the control of the Embden-Meyerhof pathway in this organism is likely to be modulated by pyrophosphate and/or polyphosphate.

引用

页码：791 / 794

页数：4

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