Determination of fetal RhD status from uncultured amniocytes

被引：7

作者：

Dildy, GA

Jackson, GM

Ward, K

机构：

[1] UNIV UTAH,SCH MED,DEPT OBSTET & GYNECOL,SALT LAKE CITY,UT 84132

[2] UNIV UTAH,SCH MED,DEPT HUMAN GENET,SALT LAKE CITY,UT 84132

来源：

OBSTETRICS AND GYNECOLOGY | 1996年 / 88卷 / 02期

关键词：

D O I：

10.1016/0029-7844(96)00143-3

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To determine the accuracy of DNA analysis for fetal RhD status using polymerase chain reaction (PCR) on uncultured amniocytes. Methods: Three hundred forty-seven amniotic fluid (AF) samples obtained for a variety of clinical indications were tested for fetal RhD status using PCR and primers specific for the genes coding for D and Cc/Ee. Fetal RhD status was confirmed by serologic methods on neonatal ford blood obtained at the time of delivery. Results: In 346 of 347 cases (99.7%), the PCR results of AF were in agreement with the serologic RhD blood type. The single error was in an RhD-negative fetus (misidentified as RhD-positive) whose AF was contaminated with blood from an RhD-positive mother. The likelihood of an erroneous result was 0.3%. Conclusion: Polymerase chain reaction-based analysis of fetal RhD status using uncultured amniocytes and established primers appears to be highly accurate.

引用

收藏

页码：207 / 210

页数：4

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