A sensitive microplate-based continuous-monitoring (kinetic) assay for serum neutral endopeptidase (EC 3.4.24.11) activity

被引：9

作者：

Selmeci, L

Szokodi, I

HorvatKarajz, K

机构：

[1] Department of Cardiovascular Surgery, Semmelweis Medical University, H-1525 Budapest

来源：

CLINICA CHIMICA ACTA | 1996年 / 244卷 / 01期

关键词：

human serum; neutral endopeptidase; kinetic enzyme assay; microplate reader;

D O I：

10.1016/0009-8981(95)06194-0

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A sensitive microplate-based continuous monitoring (kinetic) assay is described for the determination of serum neutral endopeptidase (EC 3.4.24.11) activity. The realtive simple technique is suitable for routine and serial measurements of neutral endopeptidase activity in human serum and other body fluids using a kinetic microplate reader. In 38 healthy blood donors (27 males, 11 females, age range 19-51 years) the mean activity of the enzyme was 3.43 +/- 1.02 U/l. The activities did not differ between sexes (P = 0.895, N.S.).

引用

页码：111 / 116

页数：6

共 13 条

[1]

ALMENOFF J, 1984, J LAB CLIN MED, V103, P420

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] INHIBITION OF NEUTRAL ENDOPEPTIDASE STIMULATES RENAL SODIUM-EXCRETION IN PATIENTS WITH CHRONIC-RENAL-FAILURE [J].