FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon

被引：109

作者：

Angelini, S

Deitermann, S

Koch, HG

机构：

[1] Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany

[2] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany

来源：

EMBO REPORTS | 2005年 / 6卷 / 05期

关键词：

SRP; FtsY; SRP receptor; SecYEG; co-translational targeting;

D O I：

10.1038/sj.embor.7400385

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Co-translational membrane targeting of proteins by the bacterial signal-recognition particle (SRP) requires the specific interaction of the SRP - ribosome nascent chain complex with FtsY, the bacterial SRP receptor (SR). FtsY is homologous to the SR alpha-subunit of the eukaryotic SR, which is tethered to the endoplasmic-reticulum membrane by its interaction with the integral SR beta-subunit. In contrast to SR alpha, FtsY is partly membrane associated and partly located in the cytosol. However, the mechanisms by which FtsY associates with the membrane are unclear. No gene encoding an SR beta homologue has been found in bacterial genomes, and the presence of an FtsY-specific membrane receptor has not been shown so far. We now provide evidence for the direct interaction between FtsY and the SecY translocon. This interaction offers an explanation of how the bacterial SRP cycle is regulated in response to available translocation channels.

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收藏

页码：476 / 481

页数：6

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