Fra1 activity in the frog, Rana esculenta, testis:: A new potential role in sperm transport

被引：15

作者：

Cobellis, G ^{[1
]}

Lombardi, M ^{[1
]}

Scarpa, D ^{[1
]}

Izzo, G ^{[1
]}

Fienga, G ^{[1
]}

Meccariello, R ^{[1
]}

Pierantoni, R ^{[1
]}

Fasano, S ^{[1
]}

机构：

[1] Univ Naples 2, Sez F Bottazzi, Dipartimento Med Sperimentale, I-80138 Naples, Italy

来源：

BIOLOGY OF REPRODUCTION | 2005年 / 72卷 / 05期

关键词：

AP-1; blood vessel; efferent duct; Fos family proteins; Fra1; male reproductive tract; male sexual function; peritubular myoid cell; proto-oncogenes; reproduction; spermiation; sperm motility and transport; testis;

D O I：

10.1095/biolreprod.104.036541

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Using an anti-Fos family member antibody, we have previously described in Rana esculenta testis the presence of a nuclear, 43 kDa protein that we hypothesized to be Fra1. With the assistance of an antibody against Fra1 that does not cross-react with other Fos family members, here we report data on Fra1 expression, localization, and putative activity in Rana esculenta testis during its annual reproductive cycle. Western blot analysis confirms that the nuclear, 43 kDa protein is Fra1. Immunocytochemistry validates the Western blot results and shows cytoplasmic and nuclear immunostaining of Fra1 in peritubular myoid cells, efferent ducts, and blood vessels. We report for the first time in a vertebrate, experimental evidence showing that the expression of Fra1 is related to peritubular myoid cells during sperm transport from the tubular compartment to efferent ducts.

引用

页码：1101 / 1108

页数：8

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