Using the flow cytometry to quantify the Giardia cysts and cryptosporidium oocysts in water samples

被引：17

作者：

Hsu, BM

Wu, NM ^{[1
]}

Jang, HD

Shih, FC

Wan, MT

Kung, CM

机构：

[1] Yuanpei Univ Sci & Technol, Dept Environm Engn & Hlth, Hsinchu, Taiwan

[2] Chung Yuan Christian Univ, Dept Environm Engn, Chungli 32023, Taiwan

[3] Yuanpei Univ Sci & Technol, Dept Food Sci, Hsinchu, Taiwan

[4] Yuanpei Univ Sci & Technol, Dept Med Technol, Hsinchu, Taiwan

来源：

ENVIRONMENTAL MONITORING AND ASSESSMENT | 2005年 / 104卷 / 1-3期

关键词：

Cryptosporidium; flow cytometry; Giardia; protozoan parasites;

D O I：

10.1007/s10661-005-1608-6

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The flow cytometry (FC) has been used to detect Giardia cysts and Cryptosporidium oocysts quantitatively and instantaneously in this study. The experimental results showed that FC is potential to become a more precise method for the detection of Giardia and Cryptosporidium in water. This study also evaluated the staining efficiencies for three commercial antibodies. After staining Cryptosporidium oocysts with direct immunofluorescent antibodies in water samples, two populations were detected in the scatter-plots (FLI versus SSC) of the FC. The Cryptosporidium oocysts and Giardia cysts are significantly separated from other particles while stained with direct immunofluorescent antibodies produced by Meridian Diagnostics and Waterborne™ Inc.

引用

页码：155 / 162

页数：8

共 9 条

[1] A NEW METHOD FOR EVALUATING EXPERIMENTAL CRYPTOSPORIDIAL PARASITE LOADS USING IMMUNOFLUORESCENT FLOW-CYTOMETRY [J].