Role of OsNPR1 in rice defense program as revealed by genomewide expression analysis

被引：92

作者：

Sugano, Shoji ^{[1
]}

Jiang, Chang-Jie ^{[1
]}

Miyazawa, Shin-Ichi ^{[2
]}

Masumoto, Chisato ^{[2
]}

Yazawa, Katsumi ^{[1
]}

Hayashi, Nagao ^{[1
]}

Shimono, Masaki ^{[1
]}

Nakayama, Akira ^{[1
]}

Miyao, Mitsue ^{[2
]}

Takatsuji, Hiroshi ^{[1
]}

机构：

[1] Natl Inst Agrobiol Sci, Div Plant Sci, Plant Dis Resistance Res Unit, Tsukuba, Ibaraki 3058602, Japan

[2] Natl Inst Agrobiol Sci, Div Plant Sci, Photobiol & Photosynth Res Unit, Tsukuba, Ibaraki 3058602, Japan

来源：

PLANT MOLECULAR BIOLOGY | 2010年 / 74卷 / 06期

关键词：

Benzothiadiazole; Blast resistance; OsNPR1; NPR1; Photosynthesis; Abscisic acid; SYSTEMIC ACQUIRED-RESISTANCE; SALICYLIC-ACID; DISEASE-RESISTANCE; MAGNAPORTHE-GRISEA; SIGNALING PATHWAY; GENE-EXPRESSION; SIGMA-FACTOR; ARABIDOPSIS; NPR1; PLANTS;

D O I：

10.1007/s11103-010-9695-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NPR1 is a central regulator of salicylic-acid (SA)-mediated defense signaling in Arabidopsis. Here, we report the characterization of OsNPR1, an Oryzae sativa (rice) ortholog of NPR1, focusing on its role in blast disease resistance and identification of OsNPR1-regulated genes. Blast resistance tests using OsNPR1 knockdown and over-expressing rice lines demonstrated the essential role of OsNPR1 in benzothiadiazole (BTH)-induced blast resistance. Genome-wide transcript profiling using OsNPR1-knockdown lines revealed that 358 genes out of 1,228 BTH-upregulated genes and 724 genes out of 1,069 BTH-downregulated genes were OsNPR1-dependent with respect to BTH responsiveness, thereby indicating that OsNPR1 plays a more vital role in gene downregulation. The OsNPR1-dependently downregulated genes included many of those involved in photosynthesis and in chloroplast translation and transcription. Reduction of photosynthetic activity after BTH treatment and its negation by OsNPR1 knockdown were indeed reflected in the changes in Fv/Fm values in leaves. These results imply the role of OsNPR1 in the reallocation of energy and resources during defense responses. We also examined the OsNPR1-dependence of SA-mediated suppression of ABA-induced genes.

引用

页码：549 / 562

页数：14

共 47 条

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