DLA-DRB1 polymorphisms in dogs defined by sequence-specific oligonucleotide probes (SSOP)

被引:17
作者
Kennedy, LJ
Carter, SD
Barnes, A
Bell, S
Bennett, D
Ollier, B
Thomson, W
机构
[1] Univ Manchester, Sch Epidemiol & Hlth Sci, Manchester M13 9PT, Lancs, England
[2] Univ Liverpool, Liverpool L69 3BX, Merseyside, England
[3] Univ Glasgow, Vet Clin Studies, Glasgow, Lanark, Scotland
来源
TISSUE ANTIGENS | 1999年 / 53卷 / 02期
基金
英国惠康基金;
关键词
DLA; MHC; dogs; genetics;
D O I
10.1034/j.1399-0039.1999.530209.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To date, DNA sequences for 29 dog DLA-DRB1 alleles have been reported. However, no data exists on the frequencies of these alleles within the general dog population, nor is there any indication of whether there is interbreed variation of allele distribution, We have addressed this by establishing a molecular based sequence-specific oligonucleotide probing (SSOP) method to identify all of the known broad DRB1 types and we have used this to type a random panel of dogs. A series of oligonucleotide probes were designed to detect known polymorphisms in the three DRB1 hypervariable able regions, together with two distinctive motifs in other regions of exon 2. This set of probes enabled us to assign broad DRB1 types, Two hundred and eighteen dogs were SSOP typed for DRB1. All but 4 of the published DLA-DRB1 alleles were identified in these animals. Interbreed variation in both allele distributions and allele frequencies were observed, which map be useful in the study of genetic variation between breeds. This variation also has implications for the selection of control groups for studies aimed at identifying MHC associations with disease susceptibility in the dog.
引用
收藏
页码:184 / 189
页数:6
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