Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli

被引：117

作者：

Donaghy, J

Kelly, PF

McKay, AM

机构：

[1] Dept Agr No Ireland, Food Sci Div Microbiol, Belfast BT9 5PX, Antrim, North Ireland

[2] Queens Univ Belfast, Dept Food Sci Microbiol, Belfast BT9 5PX, Antrim, North Ireland

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1998年 / 50卷 / 02期

关键词：

D O I：

10.1007/s002530051286

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of feruloyl esterase activity by Bacillus spp. and lactobacilli can be detected in an agar-plate assay. The assay involves the substitution of the main carbon source in specific agar with ethyl ferulate. A number of Bacillus spp., predominantly B. subtilis strains, were found to exhibit feruloyl esterase activity by this method. Of the examined lactobacilli, Lb. fermentum (NCFB 1751) showed the highest level of ferulic acid esterase activity. The enzyme was released from harvested cells by sonication and showed pH and temperature optima of 6.5 and 30 degrees C respectively.

引用

页码：257 / 260

页数：4

共 23 条

[1] FERULOYL AND PARA-COUMAROYL ESTERASE FROM ANAEROBIC FUNGI IN RELATION TO PLANT-CELL WALL DEGRADATION [J].