A novel protease from Entamoeba histolytica homologous to members of the family S28 of serine proteases

被引：9

作者：

Barrios-Ceballos, MP

Martínez-Gallardo, NA

Anaya-Velázquez, F

Mirelman, D

Padilla-Vaca, F ^{[1
]}

机构：

[1] Univ Guanajuato, Fac Quim, Inst Invest Biol Expt, Guanajuato 36050, Mexico

[2] IPN, Ctr Invest & Estudios Avanzados, Dept Biotecnol & Bioquim, Guanajuato 36050, Mexico

[3] Weizmann Inst Sci, Dept Biol Sci, IL-76100 Rehovot, Israel

来源：

EXPERIMENTAL PARASITOLOGY | 2005年 / 110卷 / 03期

关键词：

Suc-AAF-AMC; N-succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin; E-64,1-trans-epoxysuccinyl-L-leucilamide-(4-guanidine)-butane; Entainoeba histollytica; serine protease; family S28;

D O I：

10.1016/j.exppara.2005.02.022

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Serine proteases are one of the biologically most important and widely distributed enzyme families. A protease capable of degrading the substrate Sue-AAF-AMC was isolated from axenically grown trophozoites of Entamoeba histolytica. The enzyme was purified by ion-exchange chromatography and electroelution, and appeared on 2D-PAGE as a spot of 60 kDa and pI of 4.65. Data obtained from zymogram Suggest the active protease is present either as homodimer (1 30kDa) or homotetramer (250kDa). The optimal temperature of the enzyme was 37 degrees C, and it exhibited activity over a broad pH range. The protease was strongly inhibited by TPCK and chelating agents. The enzymatic activity was restored upon addition of calcium. BLAST analysis with the sequence of internal peptides of the protein revealed two open reading frames within the genome of E histolytica, homologous to members of the family S28, clan SC of serine proteases. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：270 / 275

页数：6

共 18 条

[1] Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica [J].