Intracellular pH in one-cell mouse embryo differs between subcellular compartments and between interphase and mitosis

被引:13
作者
Amirand, C
Mentré, P
van de Geijn, S
Waksmundzka, M
Debey, P
机构
[1] Museum Natl Hist Nat, INRA, Inst Biol Physicochim, U806, F-75005 Paris, France
[2] Univ Paris 06, CNRS, Lab Physicochim Biomol & Cellulaire, F-75005 Paris, France
[3] Univ Warsaw, Inst Zool, Dept Embryol, PL-00729 Warsaw 64, Poland
关键词
intracellular pH; one-cell mouse embryo; nuclear pH; nucleolar pH;
D O I
10.1016/S0248-4900(00)01080-7
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The pH-sensitive dual-emission fluorophore SNARF-1 coupled with a laser confocal microspectrofluorimeter was used to measure the internal pH (pH(i)) in different subcellular and subnuclear compartments of early mouse embryos. By this method we analysed the first cell cycle of naturally fertilised embryos in order to detect possible pH(i) changes correlated to cellular events, particularly the onset of replication or transcription and the first mitosis. Throughout interphase, significant differences of pH(i) were observed between cytoplasm and pronuclei, and, even more striking, between these compartments and nucleolus precursor bodies, whose pH(i) was systematically lower. We could detect significant pH(i) change neither during the replication phase nor at the onset of zygotic transcription, but pH(i) increased at the end of the one-cell stage in both cytoplasm and chromatin regions, a process that seemed specifically correlated with mitosis. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
引用
收藏
页码:409 / 419
页数:11
相关论文
共 73 条
[1]  
Adenot PG, 1997, DEVELOPMENT, V124, P4615
[2]   DYNAMICS OF PATERNAL CHROMATIN CHANGES IN LIVE ONE-CELL MOUSE EMBRYO AFTER NATURAL FERTILIZATION [J].
ADENOT, PG ;
SZOLLOSI, MS ;
GEZE, M ;
RENARD, JP ;
DEBEY, P .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1991, 28 (01) :23-34
[3]   ULTRASTRUCTURAL AND CYTOCHEMICAL STUDIES ON EXTRANUCLEOLAR BODIES IN RAT OOCYTES AT THE PREOVULATORY FOLLICLE STAGE [J].
ANTOINE, N ;
THIRY, M ;
GOESSENS, G .
BIOLOGY OF THE CELL, 1989, 65 (01) :61-66
[4]  
Baltz JM, 1997, ZYGOTE, V5, P1
[5]   INTRACELLULAR PH MEASUREMENT USING SINGLE EXCITATION-DUAL EMISSION FLUORESCENCE RATIOS [J].
BASSNETT, S ;
REINISCH, L ;
BEEBE, DC .
AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (01) :C171-C178
[6]   Early ionic events in activation of the mammalian egg [J].
Ben-Yosef, D ;
Shalgi, R .
REVIEWS OF REPRODUCTION, 1998, 3 (02) :96-103
[7]   Intracellular pH of rat eggs is not affected by fertilization and the resulting calcium oscillations [J].
BenYosef, D ;
Oron, Y ;
Shalgi, R .
BIOLOGY OF REPRODUCTION, 1996, 55 (02) :461-468
[8]  
BIGGIOGERA M, 1990, DEVELOPMENT, V110, P1263
[9]   PHYSIOLOGICALLY INACTIVE NUCLEOLI CONTAIN NUCLEOPLASMIC RIBONUCLEOPROTEINS - IMMUNOELECTRON MICROSCOPY OF MOUSE SPERMATIDS AND EARLY EMBRYOS [J].
BIGGIOGERA, M ;
MARTIN, TE ;
GORDON, J ;
AMALRIC, F ;
FAKAN, S .
EXPERIMENTAL CELL RESEARCH, 1994, 213 (01) :55-63
[10]   ENDOGENOUS TRANSCRIPTION OCCURS AT THE 1-CELL STAGE IN THE MOUSE EMBRYO [J].
BOUNIOL, C ;
NGUYEN, E ;
DEBEY, P .
EXPERIMENTAL CELL RESEARCH, 1995, 218 (01) :57-62