Inhibition of mesoderm formation by follistatin

被引：12

作者：

Marchant, L ^{[1
]}

Linker, C ^{[1
]}

Mayor, R ^{[1
]}

机构：

[1] Univ Chile, Fac Ciencias, Lab Biol Desarrollo, Santiago, Chile

来源：

DEVELOPMENT GENES AND EVOLUTION | 1998年 / 208卷 / 03期

关键词：

activin; Xenopus; follistatin; mesoderm; Vg1;

D O I：

10.1007/s004270050167

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mesoderm induction requires interaction between cells of the animal and vegetal hemispheres of the embryo. Several molecules have been proposed as candidates for mesoderm-inducing signals, with activin a particularly strong candidate. However, it has not been possible to inhibit mesoderm formation in vivo by specifically blocking activin action. Follistatin is able to inhibit the action of activin but not that of the mature region of Vg1, a member of the transforming growth factor beta family. Follistatin therefore provides a useful tool for distinguishing between signalling by these two factors. We have overexpressed Xenopus follistatin mRNA and analysed the expression of several mesodermal markers. Our results show an inhibition of mesodermal formation by follistatin in a concentration-dependent manner, showing the requirement of activin for mesodermal induction.

引用

页码：157 / 160

页数：4

共 13 条

[1] EXPRESSION OF A DOMINANT NEGATIVE MUTANT OF THE FGF RECEPTOR DISRUPTS MESODERM FORMATION IN XENOPUS EMBRYOS
AMAYA, E
MUSCI, TJ
KIRSCHNER, MW
[J]. CELL, 1991, 66 (02) : 257 - 270
[2] ARMES NA, 1997, DEVELOPMENT, V124, P3979
[3] SECRETION AND MESODERM-INDUCING ACTIVITY OF THE TGF-BETA-RELATED DOMAIN OF XENOPUS-VG1
DALE, L
MATTHEWS, G
COLMAN, A
[J]. EMBO JOURNAL, 1993, 12 (12) : 4471 - 4480
[4] Activin signalling has a necessary function in Xenopus early development
Dyson, S
Gurdon, JB
[J]. CURRENT BIOLOGY, 1997, 7 (01) : 81 - 84
[5] IDENTIFICATION OF ACTIVIN-A, ACTIVIN-AB, AND ACTIVIN-B AND FOLLISTATIN PROTEINS IN XENOPUS-EMBRYOS
FUKUI, A
NAKAMURA, T
UCHIYAMA, H
SUGINO, K
SUGINO, H
ASASHIMA, M
[J]. DEVELOPMENTAL BIOLOGY, 1994, 163 (01) : 279 - 281
[6] RESPONSES OF EMBRYONIC XENOPUS CELLS TO ACTIVIN AND FGF ARE SEPARATED BY MULTIPLE DOSE THRESHOLDS AND CORRESPOND TO DISTINCT AXES OF THE MESODERM
GREEN, JBA
NEW, HV
SMITH, JC
[J]. CELL, 1992, 71 (05) : 731 - 739
[7] FOLLISTATIN, AN ANTAGONIST OF ACTIVIN, IS EXPRESSED IN THE SPEMANN ORGANIZER AND DISPLAYS DIRECT NEURALIZING ACTIVITY
HEMMATIBRIVANLOU, A
KELLY, OG
MELTON, DA
[J]. CELL, 1994, 77 (02) : 283 - 295
[8] A TRUNCATED ACTIVIN RECEPTOR INHIBITS MESODERM INDUCTION AND FORMATION OF AXIAL STRUCTURES IN XENOPUS EMBRYOS
HEMMATIBRIVANLOU, A
MELTON, DA
[J]. NATURE, 1992, 359 (6396) : 609 - 614
[9] MAYOR R, 1995, DEVELOPMENT, V121, P767
[10] ACTIVIN-BINDING PROTEIN FROM RAT OVARY IS FOLLISTATIN
NAKAMURA, T
TAKIO, K
ETO, Y
SHIBAI, H
TITANI, K
SUGINO, H
[J]. SCIENCE, 1990, 247 (4944) : 836 - 838

← 1 2 →