Calculation of the composition of sample zones in capillary zone electrophoresis .3. Weak bases and the application of background electrolytes with two co-ions

被引:35
作者
Beckers, JL
机构
[1] Eindhoven University of Technology, Laboratory of Instrumental Analysis, 5600 MB Eindhoven
关键词
sample zone composition; background electrophoresis; mathematical model; fronting; tailing; weak bases;
D O I
10.1016/0021-9673(96)00162-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
All parameters of the sample zones in electrophoretic processes can be calculated by using a mathematical model based on the repeated application of steady-state equations, viz., the modified version of Ohm's law, electroneutrality and all mass balances. From calculations with this model, rules of thumb have been deduced to predict the fronting/tailing character of sample peaks, the question of peaks and dips, the pH and the transfer ratio for weak bases in capillary zone electrophoresis. Furthermore, the mathematical model is extended in order to calculate the parameters of sample zones in capillary zone electrophoresis by applying background electrolytes with two co-ions, through which insight can be obtained concerning mobility-selective displacement and system peaks. In applying background electrolytes with two co-ions, all concepts concerning peak/dips, transfer ratio and fronting/tailing character lose their meaning.
引用
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页码:265 / 277
页数:13
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