Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: An in vivo and in vitro study

被引:32
作者
Bals, Thomas [1 ]
Duenschede, Beatrix [1 ]
Funke, Silke [1 ]
Schuenemann, Danja [1 ]
机构
[1] Ruhr Univ Bochum, D-44780 Bochum, Germany
关键词
Signal recognition particle; Chloroplast; Protein transport; Alb3; Translocase; Light-harvesting chlorophyll-binding protein; SIGNAL RECOGNITION PARTICLE; CHLOROPLAST SRP PATHWAY; PROTEIN INTERACTIONS; BINDING; THYLAKOIDS; HOMOLOG; SYSTEM;
D O I
10.1016/j.febslet.2010.08.053
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The chloroplast signal recognition particle (cpSRP) and its receptor, cpFtsY, posttranslationally target the nuclear-encoded light-harvesting chlorophyll-binding proteins (LHCPs) to the translocase Alb3 in the thylakoid membrane. In this study, we analyzed the interplay between the cpSRP pathway components, the substrate protein LHCP and the translocase Alb3 by using in vivo and in vitro techniques. We propose that cpSRP43 is crucial for the binding of LHCP-loaded cpSRP and cpFtsY to Alb3. In addition, our data suggest that a direct interaction between Alb3 and LHCP contributes to the formation of this complex. Structured summary: MINT-7992851: Alb3 (uniprotkb:Q8LBP4) physically interacts (MI:0915) with cpSRP43 (uniprotkb:O22265) by two hybrid (MI: 0018) MINT-7992897: cpSRP43 (uniprotkb: O22265) and Alb3 (uniprotkb: Q8LBP4) physically interact (MI: 0915) by bimolecular fluorescence complementation (MI: 0809) MINT-7993251: SRP43 (uniprotkb: O22265) binds (MI: 0407) to LHCP (uniprotkb: P27490) by pull down (MI: 0096) MINT-7993207: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with ftsY (uniprotkb:O80842), LHCP (uniprotkb: P27490), SRP-54 (uniprotkb:P37106) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT-7993272: Alb3 (uniprotkb: Q8LBP4) and LHCB (uniprotkb: P27490) physically interact (MI: 0915) by bimolecular fluorescence complementation (MI: 0809) MINT-7992960: cpSRP43 (uniprotkb: O22265) binds (MI: 0407) to Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT-7993236: Alb3 (uniprotkb: Q8LBP4) binds (MI: 0407) to LHCP (uniprotkb: P27490) by pull down (MI: 0096) MINT-7993166: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with LHCP (uniprotkb: P27490) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT-7993118: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with Alb3 (uniprotkb: Q8LBP4), SRP-54 (uniprotkb: P37106) and LHCP (uniprotkb: P27490) by pull down (MI: 0096) MINT-7993046: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with ftsY (uniprotkb: O80842), SRP-54 (uniprotkb: P37106) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT-7993004: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with SRP54 (uniprotkb: P37106) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码:4138 / 4144
页数:7
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