Mapping of the Rsd contact site on the sigma 70 subunit of Escherichia coli RNA polymerase

被引：32

作者：

Jishage, M ^{[1
]}

Dasgupta, D ^{[1
]}

Ishihama, A ^{[1
]}

机构：

[1] Natl Inst Genet, Dept Mol Genet, Shizuoka 4118540, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 09期

关键词：

D O I：

10.1128/JB.183.9.2952-2956.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase sigma (70) subunit. The contact site of Rsd on sigma (70) was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron-p-bromoacetamidobenzyl EDTA and by analysis of the complex formation between Ala-substituted sigma (70) and Rsd. Results indicate that the Rsd contact site is located downstream of the promoter -35 recognition helix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core enzyme and sigma (70) contact transcription factors.

引用

页码：2952 / 2956

页数：5

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