Adoptive transfer of macrophages ameliorates renal fibrosis in mice

被引：80

作者：

Nishida, M ^{[1
]}

Okumura, Y ^{[1
]}

Fujimoto, S ^{[1
]}

Shiraishi, I ^{[1
]}

Itoi, T ^{[1
]}

Hamaoka, K ^{[1
]}

机构：

[1] Kyoto Prefectural Univ, Grad Sch Med Sci, Dept Pediat Cardiol & Nephrol, Kyoto 606, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 332卷 / 01期

基金：

日本学术振兴会;

关键词：

renal fibrosis; renal interstitium; adoptive macrophage transfer; bone marrow-derived macrophage; macrophage depletion; cyclophosphamide; unilateral ureteral obstruction; tissue repair;

D O I：

10.1016/j.bbrc.2005.04.083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We performed adoptive transfer of bone marrow-derived (BM) macrophages following pharmacological depletion of leukocytes in a mouse model of unilateral ureteral obstruction (UUO). Treatment with cyclophosphamide (CPM) caused marked decrease in the numbers of F4/80-positive interstitial macrophages as well as in peripheral blood leukocyte counts, and adoptive transfer of BM macrophages to CPM-treated mice resulted in significant increase in the numbers of interstitial macrophage both at day 5 and at day 14 after UUO. At day 5 after UUO, no significant change was observed in the degree of renal interstitial fibrosis either by treatment with CPM or with CPM + macrophage. However, at day 14 after UUO, treatment with CPM caused significant increase in the degree of interstitial fibrosis, and adoptive macrophage transfer to these mice attenuated this enhancement in renal fibrosis. Our result suggests the role of infiltrating macrophages on facilitating tissue repair at late stage of UUO. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：11 / 16

页数：6

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