Characterization of a naturally-occurring polymorphism in the UHR-1 gene encoding the putative rat prolactin-releasing peptide receptor

被引：13

作者：

Ellacott, KLJ

Donald, EL

Clarkson, P

Morten, J

Masters, D

Brennand, J

Luckman, SM

机构：

[1] Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England

[2] AstraZeneca Plc, Alderley Edge SK10 4GT, Cheshire, England

来源：

PEPTIDES | 2005年 / 26卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

UHR-1; PrRP; food intake; receptor autoradiography; genetic variation;

D O I：

10.1016/j.peptides.2004.11.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rat orphan receptor UHR-1 and its human orthologue, GPR10, were first isolated in 1995. The ligand for this receptor, prolactin-releasing peptide (PrRP), was identified in 1998 by reverse pharmacology and has subsequently been implicated in a number of physiological processes. As supported by its localization and regulation in the hypothalamus and brainstem, we have shown previously that PrRP is involved in energy homeostasis. Here we describe a naturally occurring polymorphism in the UHR-1 gene that results in an ATG to ATA change at the putative translational initiation site. The presence of the polymorphism abolished the binding of I-125 PrRP in rat brain slices but did not affect the ability of PrRP to reduce fast-induced food intake. Together this data suggest that PrRP may be exerting its feeding effects through a receptor other than UHR-1. (c) 2004 Elsevier Inc. All rights reserved.

引用

收藏

页码：675 / 681

页数：7

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