DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo

被引：47

作者：

Jabbur, JR

Huang, P

Zhang, W

机构：

[1] Univ Texas, MD Anderson Canc Ctr, Dept Pathol, Houston, TX 77030 USA

[2] Univ Texas, MD Anderson Canc Ctr, Dept Canc Biol, Houston, TX 77030 USA

[3] Univ Texas, MD Anderson Canc Ctr, Dept Clin Invest, Houston, TX 77030 USA

来源：

ONCOGENE | 2000年 / 19卷 / 54期

关键词：

phosphorylation; ionizing radiation; p53; p21;

D O I：

10.1038/sj.onc.1204017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the induction and physiological role of Ser20 phosphorylation of p53 in response to DNA damage caused by ionizing radiation (IR) or ultraviolet radiation (UV), A polyclonal antibody that specifically recognizes a p53 peptide containing phosphorylated Ser20 was generated and used to detect p53 phosphorylation at Ser20, Western blot analyses of p53 in four cell lines with this antibody revealed that the p53 protein was phosphorylated at Ser20 to a different extent after treatment with IR or UV, The phosphorylation of Ser20 of wild-type p53 correlated with enhanced induction of the p53 downstream target genes p21(WAF1/Cip1) (p21) and mdm-2. These results suggest that DNA damage-induced phosphorylation of p53 at Ser20 enhances the transactivation function of p53 for p21 and mdm-2 in vivo.

引用

页码：6203 / 6208

页数：6

共 48 条

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Ashcroft M, 1999, MOL CELL BIOL, V19, P1751

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