Formation, recognition and bioactivities of a novel G-quadruplex in the STAT3 gene

被引：15

作者：

Lin, Sen ^{[2
]}

Li, Sufang ^{[1
]}

Chen, Zan ^{[1
]}

He, Xiangwei ^{[1
]}

Zhang, Youyi ^{[1
]}

Xu, Xiaojie ^{[2
]}

Xu, Ming ^{[1
]}

Yuan, Gu ^{[2
]}

机构：

[1] Peking Univ, Inst Vasc Med, Hosp 3, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100191, Peoples R China

[2] Peking Univ, Beijing Natl Lab Mol Sci BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Dept Chem Biol,Coll Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2011年 / 21卷 / 19期

基金：

中国国家自然科学基金;

关键词：

G-Quadruplex; 3'-Flanking region; Cepharanthine; STAT3; gene; Gene regulation; CIRCULAR-DICHROISM; MASS-SPECTROMETRY; TELOMERIC DNA; TUMOR-CELLS; BINDING; STABILIZATION; INHIBITION; MECHANISMS; CONVERSION; TETRAPLEX;

D O I：

10.1016/j.bmcl.2011.07.121

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

G-Quadruplexes have been proved to exist in 5'-untranslated region (5'-UTR), promoter, intron and exon regions of many human genes. Here we report an intramolecular G-quadruplex formed by a G-rich sequence in the 3'-flanking region of signal transducers and activators of transcription 3 (STAT3) gene. The results showed that this G-rich sequence could affect the expression of STAT3. When the STAT3 G-quadruplex was stabilized by a novel non-planar ligand Cepharanthine (CEP), the decreased expression of STAT3 was observed in primary cultured cardiomyocytes. We also demonstrated that the down-regulation of STAT3 was most likely occurred at the transcriptional level. Our results provide a new clue for studying the G-quadruplex formation, recognition and function in the 3'-flanking region of gene. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：5987 / 5991

页数：5

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