Guar seed β-mannan synthase is a member of the cellulose synthase super gene family

被引：273

作者：

Dhugga, KS

Barreiro, R

Whitten, B

Stecca, K

Hazebroek, J

Randhawa, GS

Dolan, M

Kinney, AJ

Tomes, D

Nichols, S

Anderson, P

机构：

[1] Pioneer HiBred Int Inc, Crop Genet Res & Dev, Johnston, IA 50131 USA

[2] Indian Inst Technol, Dept Biosci & Biotechnol, Roorkee 247667, Uttar Pradesh, India

[3] DuPont Co Inc, Expt Stn, Crop Genet Res & Dev, Wilmington, DE 19880 USA

[4] DuPont Co Inc, Expt Stn, Cent Res & Dev, Wilmington, DE 19880 USA

来源：

SCIENCE | 2004年 / 303卷 / 5656期

关键词：

D O I：

10.1126/science.1090908

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genes for the enzymes that make plant cell wall hemicellulosic polysaccharides remain to be identified. We report here the isolation of a complementary DNA ( cDNA) clone encoding one such enzyme, mannan synthase (ManS), that makes the beta-1, 4-mannan backbone of galactomannan, a hemicellulosic storage polysaccharide in guar seed endosperm walls. The soybean somatic embryos expressing ManS cDNA contained high levels of ManS activities that localized to Golgi. Phylogenetically, ManS is closest to group A of the cellulose synthase-like (Csl) sequences from Arabidopsis and rice. Our results provide the biochemical proof for the involvement of the Csl genes in beta-glycan formation in plants.

引用

页码：363 / 366

页数：4

共 29 条

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