Efficiently differentiating vascular endothelial cells from adipose tissue-derived mesenchymal stem cells in serum-free culture

被引:82
作者
Konno, Masamitsu [2 ]
Hamazaki, Tatsuo S. [1 ]
Fukuda, Satsuki
Tokuhara, Makoto
Uchiyama, Hideho [3 ]
Okazawa, Hitoshi [2 ]
Okochi, Hitoshi
Asashima, Makoto [4 ]
机构
[1] Natl Ctr Global Hlth & Med, Res Inst, Dept Regenerat Med, Shinjuku Ku, Tokyo 1628655, Japan
[2] Tokyo Med & Dent Univ, Dept Neuropathol, Med Res Inst, Tokyo 1138510, Japan
[3] Yokohama City Univ, Int Grad Sch Arts & Sci, Yokohama, Kanagawa 2360027, Japan
[4] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci Biol, Tokyo 1538902, Japan
基金
日本科学技术振兴机构;
关键词
Adipose-derived mesenchymal stem cell; Vascular endothelial cell; Differentiation; Serum-free culture; FGF2; FIBROBLAST-GROWTH-FACTOR; STROMAL CELLS; IN-VITRO; REGENERATIVE MEDICINE; RECEPTOR; ANGIOGENESIS; ADIPOCYTES; EXPRESSION; LETHALITY; LOCATION;
D O I
10.1016/j.bbrc.2010.08.029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Adipose tissue-derived mesenchymal stem cells (ASCs) have been reported to be multipotent and to differentiate into various cell types, including osteocytes, adipocytes, chondrocytes, and neural cells. Recently, many authors have reported that ASCs are also able to differentiate into vascular endothelial cells (VECs) in vitro. However, these reports included the use of medium containing fetal bovine serum for endothelial differentiation. In the present study, we have developed a novel method for differentiating mouse ASCs into VECs under serum-free conditions. After the differentiation culture, over 80% of the cells expressed vascular endothelial-specific marker proteins and could take up low-density lipoprotein in vitro. This protocol should be helpful in clarifying the mechanisms of ASC differentiation into the VSC lineage. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:461 / 465
页数:5
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