Cytokinin-induced somatic embryogenesis and plant regeneration in Corydalis yanhusuo (Fumariaceae) -: a medicinal plant

被引:83
作者
Sagare, AP
Lee, YL
Lin, TC
Chen, CC
Tsay, HS
机构
[1] Agr Res Inst Taiwan, Dept Agron, Taichung 41301, Taiwan
[2] China Med Coll, Inst Chinese Pharmaceut Sci, Taichung 40421, Taiwan
关键词
ABA; Corydalis yanhusuo; growth retardant; medicinal plant; Papaveraceae; PEG-4000; somatic embryogenesis;
D O I
10.1016/S0168-9452(00)00377-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An efficient method has been developed for regeneration of complete plants via somatic embryogenesis in Corydalis yanhusuo (Fumariaceae), an important medicinal plant, using tuber-derived callus. Primary callus was induced by culturing mature tuber pieces on Murashige and Skoog's (MS) medium supplemented with 2.0 mg 1(-1) N-6-benzyladenine (BA) and 0.5 mg 1(-1) alpha -naphthaleneacetic acid (NAA) in darkness. Somatic embryos were induced by subculturing the primary callus on MS medium supplemented with 0.5-4.0 mg 1(-1) BA, kinetin, or zeatin, within 2 weeks of culture in light. Embryos with well-developed cotyledonary leaves were transferred in half-strength liquid MS medium supplemented with 1.0 mg 1(-1) zeatin riboside for the development of roots. Converted somatic embryos were cultured on half-strength MS medium supplemented with 6% sucrose, and with 0.5-10.0 mg 1(-1) abscisic acid (ABA), paclobutrazol, or ancymidol, 0.5-5.0 mg 1(-1) GA(3) and 15-100 mg 1(-1) polyethylene glycol (PEG) 4000 for further development of plantlets and in vitro tuber formation. The development of somatic embryos over the surface of tuber and/or cotyledonary leaf base region of the converted primary somatic embryo was observed. Before ex vitro establishment of somatic embryo-derived plants, plants with well-developed tubers were cultured on half-strength MS medium with 2% sucrose and 0.1 mg 1(-1) GA(3) for 3 weeks. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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页码:139 / 147
页数:9
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