Analysis of non-covalent protein complexes by capillary electrophoresis-time-of-flight mass spectrometry

被引：30

作者：

Brenner-Weiss, G ^{[1
]}

Kirschöfer, F ^{[1
]}

Kühl, B ^{[1
]}

Nusser, M ^{[1
]}

Obst, U ^{[1
]}

机构：

[1] Forschungszentrum Karlsruhe, Inst Tech Chem, Bereich Wasser & Geotechnol, D-76021 Karlsruhe, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1009卷 / 1-2期

关键词：

coaxial liquid sheath flow; mass spectrometry; proteins;

D O I：

10.1016/S0021-9673(03)00553-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary electrophoresis-electrospray ionisation time-of-flight mass spectrometry (CE-ESI-TOF-MS) method for characterisation of non-covalent protein complexes is described using a coaxial liquid sheath-flow sprayer. The CE capillary was connected to the mass spectrometer using a commercial CE-MS sprayer mounted on a ceramic holder of the ESI interface of the mass spectrometer. Using myoglobin (Mb) as an example of non-covalent protein complex, the effect on complex stability caused by organic modifiers added to the sheath liquid was analysed. Depending on the amount of methanol, either intact Mb or the apoprotein and the prosthetic heme group were detected. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：147 / 153

页数：7

共 9 条

[1] Recent advances in capillary electrophoresis electrospray mass spectrometry [J].