Significance of the YLDL motif in the M protein and Alix/AIP1 for Sendai virus budding in the context of virus infection

被引：17

作者：

Irie, Takashi

Inoue, Makoto ^{[2
]}

Sakaguchi, Takemasa ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Virol, Minami Ku, Hiroshima 7348551, Japan

[2] DNAVEC Corp, Tsukuba, Ibaraki 3050856, Japan

来源：

VIROLOGY | 2010年 / 405卷 / 02期

基金：

日本学术振兴会;

关键词：

Sendai virus; Budding; Alix/AIP1; Matrix protein; C-PROTEIN; BINDING PARTNER; MATRIX PROTEIN; PARTICLES; ALIX; RNA; EXPRESSION; DOMAINS; MECHANISMS; AIP1/ALIX;

D O I：

10.1016/j.virol.2010.06.031

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sendai virus (SeV) M protein has a YLDL motif, which is essential for budding of virus-like particles (VLPs) by expression of the M protein. We investigated the importance of the YLDL motif for SeV budding. Virus budding of an M-deficient SeV was not rescued by transient expression of motif mutants, M-A2 (ALDA) and M-A4 (AAAA), and viruses possessing those mutations hardly propagated in cultured cells. However, a budding-competent revertant virus, SeV M-A2R, was obtained from SeV M-A2, and nucleotide sequencing showed an ALDV sequence at the motif instead of the ALDA sequence derived from M-A2. The M-A2R protein rescued budding of an M-deficient SeV, formed VLPs when expressed with viral C protein, and restored the capacity to bind with Alix/AIP1. The results indicate that the YLDL motif is essential for efficient budding in the context of virus infection and suggest involvement of Alix/AIP1 in SeV budding. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：334 / 341

页数：8

共 30 条

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