The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation

被引：241

作者：

Rosa, A. ^{[1
]}

Ballarino, M. ^{[1
]}

Sorrentino, A. ^{[1
,4
]}

Sthandier, O. ^{[1
]}

De Angelis, F. G.

Marchioni, M.

Masella, B.

Guarini, A. ^{[3
]}

Fatica, A. ^{[1
]}

Peschle, C. ^{[4
]}

Bozzoni, I. ^{[1
,2
]}

机构：

[1] Univ Roma La Sapienza, Inst Pasteur Cenci Bolognetti, Dept Genet & Mol Biol, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Inst Mol Biol & Pathol, I-00185 Rome, Italy

[3] Univ Roma La Sapienza, Dept Cell Biotechnol & Hematol, I-00185 Rome, Italy

[4] Ist Super Sanita, Dept Hematol Oncol & Mol Med, I-00161 Rome, Italy

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 50期

关键词：

hematopoietic differentiation; microRNA; NFI-A; monocytopoiesis;

D O I：

10.1073/pnas.0706963104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a pathway by which the master transcription factor PU.1 regulates human monocyte/macrophage differentiation. This includes miR-424 and the transcriptional factor NFI-A. We show that PU.1 and these two components are interlinked in a finely tuned temporal and regulatory circuitry: PU.1 activates the transcription of miR-424, and this up-regulation is involved in stimulating monocyte differentiation through miR-424-dependent translational repression of NFI-A. In turn, the decrease in NFI-A levels is important for the activation of differentiation-specific genes such as M-CSFr. In line with these data, both RNAi against NFI-A and ectopic expression of miR-424 in precursor cells enhance monocytic differentiation, whereas the ectopic expression of NFI-A has an opposite effect. The interplay among these three components was demonstrated in myeloid cell lines as well as in human CD34+ differentiation. These data point to the important role of miR-424 and NFI-A in controlling the monocyte/macrophage differentiation program.

引用

页码：19849 / 19854

页数：6

共 30 条

[1] Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis
Akashi, K
He, X
Chen, J
Iwasaki, H
Niu, C
Steenhard, B
Zhang, JW
Haug, J
Li, LH
[J]. BLOOD, 2003, 101 (02) : 383 - 390
[2] Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters
Amendola, M
Venneri, MA
Biffi, A
Vigna, E
Naldini, L
[J]. NATURE BIOTECHNOLOGY, 2005, 23 (01) : 108 - 116
[3] 'Advanced' generation lentivirruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo
Bonci, D
Cittadini, A
Latronico, MVG
Borello, U
Aycock, JK
Drusco, A
Innocenzi, A
Follenzi, A
Lavitrano, M
Monti, MG
RossJr, J
Naldini, L
Peschle, C
Cossu, G
Condorelli, G
[J]. GENE THERAPY, 2003, 10 (08) : 630 - 636
[4] Quantitative assessment of gene targeting in vitro and in vivo by the pancreatic transcription factor, Pdx1.: Importance of chromatin structure in directing promoter binding.
Chakrabarti, SK
James, JC
Mirmira, RG
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (15) : 13286 - 13293
[5] MicroRNAs modulate hematopoietic lineage differentiation
Chen, CZ
Li, L
Lodish, HF
Bartel, DP
[J]. SCIENCE, 2004, 303 (5654) : 83 - 86
[6] CHEN HM, 1995, ONCOGENE, V11, P1549
[7] miRNAs, cancer, and stem cell division
Croce, CM
Calin, GA
[J]. CELL, 2005, 122 (01) : 6 - 7
[8] A new vector, based on the Polll promoter of the U1 snRNA gene, for the expression of siRNAs in mammalian cells
Denti, MA
Rosa, A
Sthandier, O
De Angelis, FG
Bozzoni, I
[J]. MOLECULAR THERAPY, 2004, 10 (01) : 191 - 199
[9] A minicircuitry comprised of MicroRNA-223 and transcription factors NFI-A and C/EBPα regulates human granulopoiesis
Fazi, F
Rosa, A
Fatica, A
Gelmetti, V
De Marchis, ML
Nervi, C
Bozzoni, I
[J]. CELL, 2005, 123 (05) : 819 - 831
[10] MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation
Felli, N
Fontana, L
Pelosi, E
Botta, R
Bonci, D
Facchiano, F
Liuzzi, F
Lulli, V
Morsilli, O
Santoro, S
Valtieri, M
Calin, GA
Liu, CG
Sorrentino, A
Croce, CM
Peschle, C
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (50) : 18081 - 18086

← 1 2 3 →