Subcutaneous panniculitis-like T-cell lymphoma - Clinicopathologic, immunophenotypic, and genotypic analysis of alpha/beta and gamma/delta subtypes

Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) is an uncommon cutaneous lymphoma that has been proposed as a distinct clinicopathologic entity, but studies of SPTCL are limited. We studied the clinicopathologic, immunophenotypic, and genetic features of II SPTCLs. All cases had a variable admixture of pleomorphic small, medium, or large lymphocytes and histiocytes infiltrating the subcutis in a lobular panniculitis-like pattern. A granulomatous reaction was seen in three cases and erythrophagocytosis in four. Karyorrhexis and fat necrosis were present in all cases. Angioinvasion was seen in seven SPTCLs; four had areas of coagulation necrosis. All cases expressed T-cell-associated antigens (CD3 epsilon, CD45RO, or CD43) and T-cell receptors (TCR); nine expressed alpha beta TCRs and two expressed gamma delta TCRs. T-cell receptor-gamma, TCR beta, or TCR delta genes were clonally rearranged in 8 of 10 cases studied. Both gamma delta SPTCLs expressed V(delta)2(+) TCRs and were CD4(-), CD8(-) and CD56(+). CD56 was negative in seven of nine alpha beta SPTCLs and inconclusive in the other two. Six of nine alpha beta SPTCLs were CD8(+); the CD4/CD8 phenotypes were indeterminate in the other three. Cytolytic granule-associated proteins were expressed by all SPTCLs (II of II were TIA-1(+), 4 of 4 were perforin(+)). In situ hybridization for Epstein-Barr virus-encoded RNA (EBER-1) was negative in all cases. Most patients responded to systemic chemotherapy or local radiation therapy. Seven patients are alive: four without disease (19-73 months) and three with disease (32-72 months); four died: three of disease (3-25 months) and one without disease (42 months). We conclude that SPTCLs are clonal, EBV-, cytotoxic T-cell lymphomas derived from alpha beta T-cells or gamma delta T-cells. The gamma delta SPTCLs appear to be preferentially derived from the V(delta)2(+) subset. Subcutaneous panniculitis-like T-cell lymphoma may be rapidly fatal or indolent; local therapy may be appropriate for some patients.