Simultaneous determination of nine fluoroquinolones in egg white and egg yolk by liquid chromatography with fluorescence detection

被引：96

作者：

Zeng, ZL ^{[1
]}

Dong, AG ^{[1
]}

Yang, GX ^{[1
]}

Chen, ZL ^{[1
]}

Huang, XH ^{[1
]}

机构：

[1] S China Agr Univ, Coll Vet Med, Guangdong Prov Key Lab Vet Pharmaceut Dev & Safet, Guangzhou 510642, Guangdong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 821卷 / 02期

关键词：

fluoroquinolones; LC; eggs; method;

D O I：

10.1016/j.jchromb.2005.05.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A liquid chromatographic (LC) method with fluorescence detection was developed for determination of nine fluoroquinolones (FQs) in egg white and yolk. Egg white samples were deproteinized with acidified ethanol (egg yolk samples with acetonitrile and acidified ethanol), followed by defatting with hexane once (white) or twice (yolk), and extracting FQs into acetonitrile. After acetonitrile was evaporated, the residue was dissolved in mobile phase, and FQs were detected in LC with a fluorescence detector. Recoveries for nine FQs from white and yolk were 74.7-85.6%, 79.1-91.2%, respectively, with excellent relative standard deviations. The limits of quantification were 5-20 ng g(-1). (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：202 / 209

页数：8

共 8 条

[1]

Liquid chromatographic determination of fluoroquinolones in egg albumen and egg yolk of laying hens using fluorometric detection [J].