High-throughput identification of genes promoting neuron formation and lineage choice in mouse embryonic stem cells

被引：10

作者：

Falk, Anna ^{[1
]}

Karlsson, Tobias E. ^{[1
]}

Kurdija, Sanja ^{[1
]}

Frisen, Jonas ^{[1
]}

Zupicich, Joel ^{[1
]}

机构：

[1] Karolinska Inst, Dept Cell & Mol Biol, Med Nobel Inst, S-17177 Stockholm, Sweden

来源：

STEM CELLS | 2007年 / 25卷 / 06期

关键词：

developmental biology; gene expression; fluorescence-activated cell sorting analysis; embryonic stem cells; fluorescent protein reporter genes; gene delivery systems in vivo or in vitro; stem cell culture; neural differentiation;

D O I：

10.1634/stemcells.2006-0485

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The potential of embryonic stem cells to differentiate to all cell types makes them an attractive model for development and a potential source of cells for transplantation therapies. Candidate approaches have identified individual genes and proteins that promote the differentiation of embryonic stem cells to desired fates. Here, we describe a rapid large-scale screening strategy for the identification of genes that influence the pluripotency and differentiation of embryonic stem cells to specific fates, and we use this approach to identify genes that induce neuron formation. The power of the strategy is validated by the fact that, of the 15 genes that resulted in the largest increase in neuron number, 8 have previously been implicated in neuronal differentiation or survival, whereas 7 represent novel genes or known genes not previously implicated in neuronal development. This is a simple, fast, and generally applicable strategy for the identification of genes promoting the formation of any specific cell type from embryonic stem cells.

引用

页码：1539 / 1545

页数：7

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