Immunogold localization of pectin methylesterases in the cortical tissues of flax hypocotyl

被引：49

作者：

Morvan, O ^{[1
]}

Quentin, M ^{[1
]}

Jauneau, A ^{[1
]}

Mareck, A ^{[1
]}

Morvan, C ^{[1
]}

机构：

[1] Univ Rouen, Fac Sci, Lab Parois & Polymeres Parietaux, Serv Cooperat Univ Enterprise Org Reg,CNRS ESA 60, F-76821 Mt St Aignan, France

来源：

PROTOPLASMA | 1998年 / 202卷 / 3-4期

关键词：

epidermis; flax; pectin; pectin methylesterase; plasmalemma; wall microdomains;

D O I：

10.1007/BF01282545

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Pectin methylesterases (PMEs, EC 3.1.1.11) catalyse the deesterification of pectins. Up to now, most information concerning their location was obtained from biochemical analyses. Taking advantage of specific anti-PME antibodies, we report the precise localization of PMEs at the electron microscopy level within the different cortical tissues of flax hypocotyl. Quantitative data on the densities of immunolabelling have been collected, using anti-PME antibodies as well as JIM5 and JIM7 monoclonal antibodies. Our findings show a co-localization of PMEs and acidic pectins (as revealed by JIM5 antibodies) within specific cell wall microdomains. Moreover, PME epitopes are associated with the cellular membranes, particularly with the plasmalemma.

引用

页码：175 / 184

页数：10

共 37 条

[31] ELECTROSTATIC EFFECTS AND THE DYNAMICS OF ENZYME-REACTIONS AT THE SURFACE OF PLANT-CELLS .1. A THEORY OF THE IONIC CONTROL OF A COMPLEX MULTIENZYME SYSTEM [J].

RICARD, J ;

NOAT, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 155 (01) :183-190

[32]

RIHOUEY C, 1995, PLANT PHYSIOL BIOCH, V33, P497

[33]

SCHAUMANN A, 1993, PLANT CELL PHYSIOL, V34, P891

[34]

Thiery JP, 1967, J MICROSCOPIE, V6, P927

[35]

VANNIER MP, 1994, THESIS U ROUEN MONT

[36]

VERSTEEG C, 1978, LEBENSM WISS TECHNOL, V11, P267

[37] COLOCALIZATION OF THE CELLULOSE FRAMEWORK AND CELL-WALL MATRIX IN HELICOIDAL CONSTRUCTIONS [J].

VIAN, B ;

TEMSAH, M ;

REIS, D ;

ROLAND, JC .

JOURNAL OF MICROSCOPY, 1992, 166 :111-122

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