Effects of Ser130Gly and Asp240Lys substitutions in extended-spectrum β-lactamase CTX-M-9

被引：15

作者：

Aumeran, C

Chanal, C

Labia, R

Sirot, D

Sirot, J

Bonnet, R

机构：

[1] Fac Med, Serv Bacteriol Virol, Bacteriol Lab, F-63001 Clermont Ferrand, France

[2] MNHN, UMR 175, CNRS, F-29000 Quimper, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 09期

关键词：

D O I：

10.1128/AAC.47.9.2958-2961.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In CTX-M-9 extended-spectrum beta-lactamases (ESBLs), an S130G mutation induced a 40- to 650-fold increase in 50% inhibitory concentrations but decreased hydrolytic activity against cefotaxime. A D240K mutation did not modify enzymatic efficiency against ceftazidime. Residue K240 could interact with Q270 and. therefore not with ceftazidime, in contrast with what was observed with certain TEM/SHV-type ESBLs.

引用

页码：2958 / 2961

页数：4

共 23 条

[1] Ceftazidime-hydrolysing CTX-M-15 extended-spectrum β-lactamase (ESBL) in Poland
Baraniak, A
Fiett, J
Hryniewicz, W
Nordmann, P
Gniadkowski, M
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2002, 50 (03) : 393 - 396
[2] CLOSE AMINO-ACID-SEQUENCE RELATIONSHIP BETWEEN THE NEW PLASMID-MEDIATED EXTENDED-SPECTRUM BETA-LACTAMASE-BULLET-MEN-1 AND CHROMOSOMALLY ENCODED ENZYMES OF KLEBSIELLA-OXYTOCA
BARTHELEMY, M
PEDUZZI, J
BERNARD, H
TANCREDE, C
LABIA, R
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1122 (01) : 15 - 22
[3] A NEW PLASMIDIC CEFOTAXIMASE FROM PATIENTS INFECTED WITH SALMONELLA-TYPHIMURIUM
BAUERNFEIND, A
CASELLAS, JM
GOLDBERG, M
HOLLEY, M
JUNGWIRTH, R
MANGOLD, P
ROHNISCH, T
SCHWEIGHART, S
WILHELM, R
[J]. INFECTION, 1992, 20 (03) : 158 - 163
[4] Molecular characterization of TEM-59 (IRT-17), a novel inhibitor-resistant TEM-derived β-lactamase in a clinical isolate of Klebsiella oxytoca
Bermudes, H
Jude, F
Chaibi, EB
Arpin, C
Bebear, C
Labia, R
Quentin, C
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) : 1657 - 1661
[5] A novel CTX-M β-lactamase (CTX-M-8) in cefotaxime-resistant Enterobacteriaceae isolated in Brazil
Bonnet, R
Sampaio, JLM
Labia, R
De Champs, C
Sirot, D
Chanal, C
Sirot, J
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (07) : 1936 - 1942
[6] Novel cefotaximase (CTX-M-16) with increased catalytic efficiency due to substitution Asp-240→Gly
Bonnet, R
Dutour, C
Sampaio, JLM
Chanal, C
Sirot, D
Labia, R
De Champs, C
Sirot, J
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (08) : 2269 - 2275
[7] Inhibition of TEM-2 beta-lactamase from Escherichia coli by clavulanic acid: Observation of intermediates by electrospray ionization mass spectrometry
Brown, RPA
Aplin, RT
Schofield, CJ
[J]. BIOCHEMISTRY, 1996, 35 (38) : 12421 - 12432
[8] Inhibitor-resistant TEM β-lactamases:: phenotypic, genetic and biochemical characteristics
Chaïbi, EB
Sirot, D
Paul, G
Labia, R
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (04) : 447 - 458
[9] A 2ND GENERATION FORCE-FIELD FOR THE SIMULATION OF PROTEINS, NUCLEIC-ACIDS, AND ORGANIC-MOLECULES
CORNELL, WD
CIEPLAK, P
BAYLY, CI
GOULD, IR
MERZ, KM
FERGUSON, DM
SPELLMEYER, DC
FOX, T
CALDWELL, JW
KOLLMAN, PA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (19) : 5179 - 5197
[10] HULETSKY A, 1993, J BIOL CHEM, V268, P3690

← 1 2 3 →