Quantification of Epstein-Barr virus-DNA load in lung transplant recipients: A comparison of plasma versus whole blood

被引：19

作者：

Bakker, Nicolaas A. ^{[1
]}

Verschuuren, Erik A. ^{[2
]}

Veeger, Nic J. ^{[3
]}

van der Bij, Wim ^{[2
]}

van Imhoff, Gustaaf W. ^{[1
]}

Kallenberg, Cees G. ^{[4
]}

Hepkema, Bouke G. ^{[5
]}

机构：

[1] Univ Groningen, Univ Med Ctr Groningen, Dept Hematol, NL-9700 RB Groningen, Netherlands

[2] Univ Groningen, Univ Med Ctr Groningen, Dept Pulm Dis, NL-9700 RB Groningen, Netherlands

[3] Univ Groningen, Univ Med Ctr Groningen, Dept Clin Epidemiol, NL-9700 RB Groningen, Netherlands

[4] Univ Groningen, Univ Med Ctr Groningen, Dept Clin Immunol, NL-9700 RB Groningen, Netherlands

[5] Univ Groningen, Univ Med Ctr Groningen, Dept Pathol & Lab Med, NL-9700 RB Groningen, Netherlands

来源：

JOURNAL OF HEART AND LUNG TRANSPLANTATION | 2008年 / 27卷 / 01期

关键词：

D O I：

10.1016/j.healun.2007.10.008

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Monitoring of the Epstein-Barr virus-DNA load is frequently used to identify patients at risk for post-transplant lymphoproliferative disease (PTLD). Epstein-Barr virus DNA can be measured in the plasma and whole blood serum compartments. Methods: We compared levels of Epstein-Barr virus DNA in whole blood and plasma using a real-time TaqMan polymerase chain reaction assay in 100 consecutive paired whole blood and plasma samples from 25 lung and heart-lung transplant recipients with detectable whole blood Epstein-Barr virus-DNA load (>2,000 copies/ml). Results: A correlation (r(2)) of 0.58 (p < 0.001).was observed between both measurements, whereas of the positive whole blood samples (>2,000 copies/ml), only 17 samples (189%) were also positive in plasma. Conclusion: These results indicate that by virtue of its sensitivity, whole blood rather than plasma may be the preferable specimen for the detection of Epstein-Barr virus DNA in lung transplant recipients.

引用

页码：7 / 10

页数：4

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